Fundamentals and applications of single-molecule FRET in biophysical research
l processes such as the temporal coupling of ligand binding and conformational changes in proteins[3]. Finally, I will show how single-molecule fluorescence studies, which are typically conducted in optical [...] laboratories, can become possible at the biochemistry bench. For this we introduce a compact and versatile 3D-printed microscopy platform that allows to assemble many different fluorescence imaging modalities [...] [1] Lerner et al., Science 359 (2018) eaan1133 [2] Agam et al., Nature Methods 20 (2023) 523-535 [3] Han et al., eLife (2024) in press [4] Moya et al., Science Advances 10 (2024) ado3427 …
